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experimental modulation of tnfa levels  (Inserm Transfert)

 
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    Inserm Transfert experimental modulation of tnfa levels
    Experimental Modulation Of Tnfa Levels, supplied by Inserm Transfert, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Article Title: Effects of inter-alpha-inhibitor in experimental endotoxic shock and disseminated intravascular coagulation.
    Article Snippet: We investigated the effects of human intera -inhibitor (I a I) on hemodynamics, oxygenation, and coagulation parameters in a porcine model of endotoxic shock.. Four groups of six animals were studied: ( 1 ) control, ( 2 ) I a I group receiving 30 mg/kg I a I over 30 min, ( 3 ) LPS group receiving 5 m g ? kg/min Escherichia coli endotoxin over 30 min, and ( 4 ) LPS 1 I a I group receiving 30 min after endotoxin 30 mg/kg/30 min I a I.. We measured hemodynamic and oxygenation parameters, usual coagulation markers and plasma levels of thrombin-antithrombin complexes, antithrombin III activity, plasminogen activator tissue type, plasminogen activator inhibitor type 1, von Willebrand factor, tumor necrosis factora , and I a I at baseline and at 30, 60, 90, 120, 180, 240, and 300 min.



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    Analysis of bronchoalveolar lavage (BAL) cell counts and protein levels for samples collected following saline and LPS administration at day 7. (a) BAL total counts, (b) BAL total neutrophil counts, (c) BAL total protein content, (d) BAL NE activity. (e) BAL TNF‐α levels. n = 6 saline ( n = 3 saline for TNF‐α), n = 7 LPS. Bars represent SEM. * p ‐value < 0.05. ** p ‐value < 0.01, **** p ‐value < 0.0001.

    Journal: Physiological Reports

    Article Title: Ferret acute lung injury model induced by repeated nebulized lipopolysaccharide administration

    doi: 10.14814/phy2.15400

    Figure Lengend Snippet: Analysis of bronchoalveolar lavage (BAL) cell counts and protein levels for samples collected following saline and LPS administration at day 7. (a) BAL total counts, (b) BAL total neutrophil counts, (c) BAL total protein content, (d) BAL NE activity. (e) BAL TNF‐α levels. n = 6 saline ( n = 3 saline for TNF‐α), n = 7 LPS. Bars represent SEM. * p ‐value < 0.05. ** p ‐value < 0.01, **** p ‐value < 0.0001.

    Article Snippet: TNF‐α levels were measured in BAL samples using the Ferret TNF‐alpha/TNFA ELISA Pair Set (Sino Biological).

    Techniques: Activity Assay